WebATP Kit HS is the right one for your application, please check the Product Selection Guide at www.biothema.com (click Products/Kits/Product selection guide). Instruments . All living cells contain ATP where it plays the role of energy currency between different cellular processes. When cells die of natural causes, ATP is normally degraded. WebApr 19, 2016 · Im purifing T cells and analizing the activity of surface ATPases, so I culture 50.000 cells for 10, 45 and 120 minutes with different concentrations of ATP and then analyze how much non-degraded ...
BioThema AB Profile
WebFeb 8, 2024 · Autocrine activation of macrophages is modulated by Connexin-43-mediated ATP release in response to TLR-4 and -2 agonists in a P2Y1-dependent manner, ultimately determining sepsis survival. ... ATP Reagent SL: BioThema: 144–041: Commercial assay: Resazurin assay: Sigma-Aldrich: 199303: ... Cell-specific conditional MAC-CX43 KO … WebCellular ATP Kit, Nov. 2004 . SE-136 40 Haninge Fax: +46 (0)8 777 00 21 Sweden E-mail: [email protected] ... Sweden E-mail: [email protected] VAT No. SE556303345401 Website: www.biothema.com . ATP Biomass Kit HS Prod. No. 266-311 . Instructions for use . Intended use . This kit is intended for determination of total ATP (adenosine … raycodex auto weight da hood
Luciferase Assay System Protocol - Promega
WebJun 24, 2024 · Luminescence assays differ from fluorescence assays because no light is directed at the sample to generate a fluorescence signal. Instead light is produced as a by-product of an enzyme-substrate reaction. For example, a luciferase enzyme, in the presence of ATP, converts a substrate (e.g. D-Luciferin) to a luminescent product. WebBioThema - Cellular ATP. Cellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited. That makes the ... WebCells were treated with extracts for 48 h and, to assess cytotoxic effects, the mass of viable cells was determined as the level of ATP using Cellular ATP Kit HTS (Biothema, Handen, Sweden). raycodex 2021 pastbin