Bin2 point mutation

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August undefined, 2024

WebMar 3, 2024 · A point mutation in BnaC01.BIN2 leads to a dwarf phenotype, and heterozygosity in the BnDF4 allele leads to short stature without marked differences in … WebDec 22, 2013 · All point mutants of BIN2 (bin2-1/BIN2 E263K, BIN2 K69R) and ARF7 (ARF7 S698/707A) were generated using the QuikChange Site-Directed Mutagenesis kit (Stratagene) according to the manufacturer’s ...

BIN2 , a New Brassinosteroid-Insensitive Locus in …

WebDec 29, 2024 · To further determine the impact of the mutation on the BR signaling outputs, we expressed native promoter-driven genomic BIN2 and the corresponding mutated variants in Col-0. These mutations led to a larger plant stature (Figs 5d, S8) and the accumulation of more unphosphorylated BES1 compared with the plants expressing wild-type BIN2 (Fig. … WebSep 24, 2024 · TOR is required for BL-mediated hypocotyl growth. Previous studies suggested that the TOR and BR signaling pathways are coordinated, as TOR regulates the BR signaling components BIN2 [Citation 32] and BZR1 [Citation 33].BRs regulate the expression of thousands of genes through transcription factors including BES1 and … helping hand washing machine lint traps

Adenosine monophosphate deaminase modulates BIN2 activity …

WebJul 11, 2002 · BIN2 encodes a member of the glycogen synthase kinase-3 (GSK3)-like kinases ( 14, 15 ). The increased kinase activity by the semidominant bin2–1 mutation … Webdet2-1 and BR-insensitive mutant bin2-1 are hypersensitive to salinity stress during seed germination and/or the seed-ling growth stage. Moreover, the hypersensitivity corre … WebBIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling GLYCOGEN SYNTHASE KINASE3 (GSK3) is a highly … helping hand welfare society

BIN2 , a New Brassinosteroid-Insensitive Locus in Arabidopsis

A gain-of-function mutation in Brassinosteroid-insensitive 2 alters ...

WebA gain-of-function mutation in Brassinosteroid-insensitive 2 alters Arabidopsis floral organ development by altering auxin levels Auxin can alter the fertility of bin2-1 plants and depends on the expression of SHY2. Brassinosteroids (BRs) play important roles in plant growth and developmental processes. WebJan 30, 2024 · For generation the mutant forms of BIN2 and CO, their coding sequences were first cloned into the pQBV3 vector, and then the point mutations were introduced by the specifically designed primers as previously described ( Zheng et al., 2004 ), following the PCR amplification program: preheating at 94°C for 3 min, 16 cycles of 94°C for 1 min, … helping hand whyallaWebDetails. geom_variant uses ggplot2::geom_point under the hood. As a result, different aesthetics such as alpha, size, color, etc. can be called upon to modify the data visualization. #' the function gggenomes::read_feats is able to read VCF files and converts them into a format that is applicable within the gggenomes' track system. Keep in mind: … helping hand website

"WebGenetic analysis of the bin2 (BR-INSENSITIVE 2) mutant indicated that the BR-insensitive dwarf phenotype was due to a semidominant mutation in the BIN2 gene that mapped … " - Bin2 point mutation

Bin2 point mutation

Full article: Brassinosteroids modulate autophagy through ...

WebApr 11, 2024 · Michel De Jaeghere, ancien directeur de la rédaction de l’excellent magazine, "Le Spectacle du monde", et aujourd’hui, responsable des numéros spéciaux du Figaro (le Figaro Histoire, etc.), membre correspondant de l’Académie des sciences morales et politiques, est l’auteur de plusieurs ouvrages dans lesquels il met sa très grande … WebJan 30, 2024 · For generation the mutant forms of BIN2 and CO, their coding sequences were first cloned into the pQBV3 vector, and then the point mutations were introduced …

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WebNov 1, 2002 · BIN2, which is identical to the UCU1 gene implicated in leaf development ( Pérez-Pérez et al., 2002 ), encodes an intracellular Ser/Thr kinase that displays significant sequence identity to the mammalian GSK3 and fruitfly ( Drosophila melanogaster) SHAGGY kinases ( Li and Nam, 2002 ). WebMar 5, 2024 · Point Mutations. A point mutation is a change in a single nucleotide in DNA. This type of mutation is usually less serious than a chromosomal alteration. An example of a point mutation is a mutation …

WebJan 5, 2024 · Encodes BIN2, a member of the ATSK (shaggy-like kinase) family. BIN2 functions in the cross-talk between auxin and brassinosteroid signaling pathways. BIN2 … WebNational Center for Biotechnology Information

WebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated protein of TOR 1B), a... http://sourcedb.ib.cas.cn/cn/ibthesis/201003/P020100329363315667749.pdf

WebAug 1, 2016 · BIN2 acts as a novel downstream effector of TOR signaling to negatively regulate photoautotrophic growth. To identify the direct partner of AtS6K2, yeast two-hybrid screening was performed to identify the putative proteins that bind to the given … New Phytologist Foundation - Wiley Online Library

WebMar 27, 2024 · HY5 L137D mutation reduces BIN2 activity. To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2 … helping hand woollahraWebNov 1, 2002 · As expected, the bin2-1-mutated BIN2 protein showed a higher kinase activity toward BES1 or BZR1 than the wild-type BIN2, whereas no phosphorylation by the wild … helping hand westborough maWebDec 22, 2013 · The dwf12-1D-gof and bin2-1-gof mutations enhance the DNA binding of ARF7 and ARF19 to the LBD16 and LBD29 promoter … helping hand wilmingtonWebDec 9, 2024 · Key message Auxin can alter the fertility of bin2-1 plants and depends on the expression of SHY2. Abstract Brassinosteroids (BRs) play important roles in plant growth and developmental processes. By systematically evaluating the phenotypes of BR biosynthesis and BR signaling mutants, researchers have reported that BRs positively … helping hand wilmington ncWebAug 25, 2016 · In the BR signaling pathway, the negative regulator BIN2 is one of 10 glycogen synthase kinase 3 (GSK3)-like kinases (GSK3s) encoded in the A. … helping hand wichita ksWebNov 9, 2024 · The gain-of-function mutant bin2-1 contains an E263K mutation in the catalytic domain of BIN2 and is semidominant, with the homozygous mutant displaying severe growth defects (Li et al., 2001 ). The heterozygous bin2-1 mutant ( bin2-1 HT) was hypersensitive to salt stress ( Figures 2 C and 2E). helping hand with heartWebMar 27, 2024 · HY5 L137D mutation reduces BIN2 activity. To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2-interacting sites in the HY5 LZ region. helping hand worldwide