WebDec 29, 2024 · BIN2 mutant expression assay The native promoter-driven genomic BIN2 gene (4058 kb) and the corresponding mutated genes were fused with the YFP tag and transferred into a Col-0 background. The … To further identify the mechanism by which HY5 regulated BIN2 in the light to destabilize BZR1, we first checked whether HY5 regulated the mRNA and/or protein levels of BIN2. As shown in Supplementary Fig. 5, both the mRNA and protein levels of BIN2 were similar in hy5 and WT, suggesting that HY5 does not … See more To investigate the cross-talk between light and BR signaling, we performed yeast two-hybrid analyses and found that BIN2, the key negative … See more Next, we investigated whether HY5 could genetically interact with BIN2 by crossing hy5 into bin2-1, a gain-of-function mutant of BIN228. We found that the hypocotyls of double mutant … See more To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2-interacting sites in the HY5 LZ region. As shown in Fig. 5a, changing HY5 Leu137 (L137) to Asp (named HY5-M) … See more Previous reports have suggested that HY5 and BIN2 have similar effects on BZR1 protein accumulation20,27. To gain further insight into their … See more
POLAR-guided signalling complex assembly and localization …
WebNov 19, 2012 · BIN2 is a negative regulator in the BR pathway, which phosphorylates and inhibits BES1 and BZR1 (4, 48–50). BIN2 belongs to glycogen synthase kinase 3 … WebBIN2 functions in the cross-talk between auxin and brassinosteroid signaling pathways. BIN2 regulates root epidermal cell fate specification by phosphorylating EGL3 and TTG1. BIN2-mediated phosphorylation appears to promote BZR1 export from the nucleus. KIB1 interacts with BIN2 blocking its interaction with substrates and promotes BIN2 degradation. gunther wwe gf
BIN2 functions redundantly with other Arabidopsis GSK3 …
WebJun 6, 2024 · Considering the BIN2 inhibitor bikinin greatly increased SA-induced gene expression (Figure 1 D–1F and Supplemental Figure 1 B–1D), we examined the functional involvement of BIN2 in SA signaling.The bin2-1 gain-of-function mutant displayed reduced sensitivity to SA in a bacterial growth assay (Figure 2 B). Similarly, the inhibitory effect of … WebApr 11, 2024 · Highlights. As a major environmental factor impacting the seasonal growth and geographical distribution of plants, temperature change significantly affects crop quality and productivity. Ethylene is a gaseous hormone with an important role in plant growth, development, and multiple stress responses, including heat and cold. Temperature stress ... WebThus, BIN2 is a good candidate to regulate PIF4 levels, as inactivation of this kinase by BL may result in the fastest-migrating form of this factor seen in BL-treated seedlings ( Fig. 1C ). A direct interaction of the full-length BIN2 protein and PIF4 was actually observed in yeast two-hybrid assays ( Fig. 2A ). gupta clothing